MSDOS compatibility? You know, that's a very good question; I honestly don't know. I want to say between "maybe" and "yes" given the card supports Wink2. So I'm guessing it's addressed via legacy BIOS resources all the same.
If the MB supports it, get the cheaper header adapter as mention before. I like that idea better. Also less chance of a hardware resource conflict.
If you absolutely must use DOS, check into FreeDOS. It's over 20 years old and was last updated in 2016. I'm think FreeDOS might be better as it has far better support for modern hardware than say MSDOS v6.22