Asus ROG Strix B250f will it fit with my gpu ?

There are no incompatibilities between that GPU and your motherboard. The GPU has a PCIe3.0 x16 interface and your motherboard has a PCIe3.0 x16 slot. Just plug it in there.

I'd be more concerned with the GPU fitting your case and your B250F motherboard supporting the CPU.
 


500 dollars. I'm about to buy ROG Strix B250F and i5-6400 LGA 1151... Also corsair vengeance 16Gb. My Current specs are H81M-D motherboard, i5-4660 LGA 1150. Im upgrading to DDR4
 
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Thanks for your help