Can 2 4gb ram sticks be put in slots 2 and 3 on a motherboard?

[iiNovaYT]

So one of my ram sticks in my motherboard is not reading so I want to change the ram slot it is in. Can 2 4gb ram sticks be put in say slots 1 and 2 or 3 and 4 or does that not work?

 

[alexoiu]

It would work, but not in dual channel.

 

[Tradesman1]

What slots are they in? May have a bad channel, or if using a third party CPU cooler might be throwing off the MC if too tight in a corner or side