Question Does it matter whether i put my graphics card in the first or second pcie x16 slot? ie does it give less performance in the second

Feb 21, 2019
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I have a 1060 6GB and an Asrock b360m pro 4 motherboard.

i was just wondering if it made any difference i put my graphics card in the second slot which is where i have it right now. when i was installing it in the first one i believe that a small part on the motherboard is blocking the graphics card from going in the whole way and therefore not putting out any signal. when i put it into the second slot it worked fine. just wondering if there were any performance differences

Thanks in advance