Does it matter which PCI-E slot I use?

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I have an ASRock P67 Extreme4 Gen3 motherboard. It has 2 PCI-E 3.0 x16 slots. I only have one graphics card at the moment (R6950). Does it matter which slot I install this card to? The motherboard recommends the top one, but access to the SATA ports is limited on this one. Can I move it to the next one down without any problems? Also, just out of interest could I install this card on a PCI-E 2.0 slot? If yes, would I get reduced performance?
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Yes, technically there are no cards released for pcie 3.0 yet. Furthermore.. i don't think anything really max out the bandwidth on 2.0/2.1 either. You will be fine using pcie 2.0 and all the cards are pretty much backward compatible on the pcie slot. I would at least make sure you're using on the x16.
 
Thanks. Interestingly I have a PCI-E 2.0 slot which is also x16

Surprised my high end card doesn't utilise the 3.0 bandwith. I should've got a cheaper motherboard!