Your mobo is an 1150 so you are limited to dual channel mode (which you can run 4 sticks in dual channel) so 2x8GB is slightly preferred from a performance viewpoint as well as is less stress on the MC (memory controller)
When you shut the system down, anything in DRAM is gone, the DRAM only has data when rig is powered up, it's not like a hard drive where data is stored even when the rig is shut down 😉