Gaming from secondary internal drive?

[gtgm_110]

I have a 1TB HDD that I use for Windows, and I have a 500GB HDD that is formatted to NTFS, but is empty. Would I have any performance loss by playing games installed from that secondary drive? Both of my drives are 6 GB/s and are in the primary Sata slots on my MOBO

 

[Eduello]

You won't notice a difference if they both spin equally fast.