Do really got to say, this kind of test should have been done on both a Intel and AMD based platform. Especially the x8/x8 and x16/x4 test. For that matter, would also have been good to see not just higher end cards used, but also cards like the GF 460 and HD 5770, so that GPU performance can be factored into how much bandwith is needed per card per pci-e slot.
That way it could be shown whether or not the lower end cards also take just as much of a performance hit at x8 and x4 as the higher end cards do, or if they do not get held back nearly as band. Reason I say this is that prior test have been done on prior graphics cards before using x4 and also x16/x4 dual card modes, and while showing a performance impact, it was no where near as bad as what you have shown. One would definitely assume that the newer generation higher end cards are indeed going to demand more from their pci-slots, as they can do more. But is this ultimately the same case (as of now anyway) with the more mainstream cards from the Geforce 450, 460, 550 line, along with the Hd 5770, 5830, 6790, and 6850 line?
Ultimately many factors are going to come to play. One is the games themselves. F1 2010 and Just Cause 2 for example took major performance hits in your test, even in Crossfire x16/x4. The other titles seemed to take less of a impact however, depending on AA settings, ect, with fps loss of maybe 4-7 fps depending on said title, showing that performance loss will definitely vary per title, with some titles still benefiting quite well from Crossfire regardless of the second card running at x4.
Another factor, as stated, is what platform is being used. You did not include any AMD based platform in this test, nor any more commonly used mainstream cards. Due to this the scope of your test was far too controlled by limiting factors, and only provided quite limited results that honestly can not speak for every x16 through x4 configuration, let alone every x8/x8 and x16/x4 dual card configuration, even with a margin of error provided. In other words, this is hardly what I would consider the end all of articles on pci-e scaling explored. Sorry Tom, on this one, no dice.