Look at the wires going to a molex and you'll see that the wires are red, yellow and 2 black. One is +12v (red I think, but I'm not looking at one so it could be yellow), the other color is +5v, and the 2 black are grounds - one for 5v and one for 12v (the PSU label should tell you which for sure).
If you were to ground your +12v line to the -12v line, you'd have a net of 24v and likely blow your HDD motor.
Mike.