The PCIe layout of the motherboard uses x16, x8/x8 and x8/x4/x4 when the full length slots are in use, with four x1 PCIe slots from the chipset added in for good measure. Note that if a device is installed into the bottom PCIe slot then SLI will not work, as the first two main PCIe slots will then run at x8/x4, and SLI needs x8/x8 minimum to work.
http://www.anandtech.com/show/8582/msi-z97-gaming-5-motherboard-review-five-is-alive
you can use them all but you have to start sharing of pcie lanes the slots only have 16 from the cpu divided up
one card / device on it is x16 or 8x4x4 or 8x8 ??
then the slots that run off the chipset same thing as you see from the chart
http://www.intel.com/content/www/us/en/chipsets/performance-chipsets/z97-chipset.html
then spacing ?? put a card in a slot that may block slots and then cant be used anyway ??
look at motherboards that have a ''PLX'' chip that sounds like more of what you need for multi slot devices ??
kinda old but gives a idea
Conclusion: PLX 8747 and Multi-GPU Setups
http://www.anandtech.com/show/6170/four-multigpu-z77-boards-from-280350-plx-pex-8747-featuring-gigabyte-asrock-ecs-and-evga/31
thing is if the device need 8x and you got slots filled and got that cut down to x4 ??
with intel the manufacture can decide on how the slots are wired and shared all you can count on it the one primary slot will never fall below x8 [ main single gpu card slot ]
now AMD is all through the chipset you start with like 42 lanes and share down and why like with 2 cards they can stay at 2 full x16 with out affecting the rest of the minor slots as dramatic
''Two of the PCI Express x16 slots (PCIE2 and PCIE3) always work at x16 speed, and the other slot (PCIE5) works at x4. ''
Read more at
http://www.hardwaresecrets.com/asrock-fatal1ty-990fx-killer-motherboard/2/#yvmzEhVOBmYHRD0c.99
or move up to a intel ''X'' board with a 40 lane CPU ? [and then a plx chip on top of that ]
Socket LGA2011-v3 processors have a maximum of 40 or 28 PCI Express 3.0 lanes for video cards
Read more at
http://www.hardwaresecrets.com/asrock-x99-extreme63-1-motherboard/2/#0Ytl2PKogmczk4Vo.99
then the catch 22's you may find on some boards ?/
There is also a PCI Express 2.0 x4 slot and one PCI Express 2.0 x1 slot. The PCI Express 2.0 x4 slot shares bandwidth with one of the SATA Express connectors, two USB 3.0 ports, and the PCI Express x1 slot; if a PCI Express x4 card is installed, all of those features will be disabled. If a PCI Express x1 or x2 card is installed, only the SATA Express interface is disabled.
Read more at
http://www.hardwaresecrets.com/asus-rampage-v-extreme-motherboard/2/#jUVp6xzUSwb7EpI0.99
When installing dual-slot video cards, you “kill” the slot immediately to the left of the slot being used (looking at the motherboard with its rear connectors facing up). This means that you can’t install a dual-slot video card in the second (PCIE3) PCI Express x16 slot, because otherwise you will block the third (PCIE4) slot, which is faster than the second. Therefore, even though this motherboard has four PCI Express x16 slots, only three of them can be used with dual-slot video cards.
Read more at
http://www.hardwaresecrets.com/asrock-990fx-extreme9-motherboard/2/#GcGqFRhVckSidTbA.99
a lot to look at and over when selecting a board to cover all your needs seems like a good place to ask this is at a mining site where guys load up gpu's to do bit-coin mining that would know just what to look at