Just for anyone else who is looking to do this later. With my particular case (Define R6), I was able to mount the MB first because there is a hole to access the back of the MB after mounting. I ran into a different kind of problem though. I installed my memory (G.Skill Trident Z RGB) but once that was done, I was unable to mount the CPU cooler. At least, not in the horizontal configuration I wanted. The only way it would fit was vertical. Not ideal but on this build, I am using fans at the top of the case blowing out so with the NH-D15 sitting vertical, perhaps some of that heat will rise. Maybe not. Can't tell with the two fans installed, exactly where all that heat is going to go. There is only one fan on the back but I have two fans at the bottom pushing up, two fans at the front pushing back and then as I mentioned, two fans at the top pushing out. Here's hoping!