It depends what you are doing and what CPU you have.
Two sticks should run in Dual Channel mode thus giving you higher bandwidth (2x higher with 2x2GB) but you may need more memory rather than higher bandwidth.
So... hard to say.
Without knowing more I'd go with running two sticks (2+4) to have more memory though as they are different you may or may not have issues.
Run Memtest86 www.memtest86.com to check for issues