Yes, there will be a difference.
On the 144 Hz monitor, it will display a new frame 2 out of 3 times the monitor refreshes - resulting in you actually seeing about 96 fps, with a little bit of tearing without VSync enabled. With VSync, you'd get to see 72 fps (as you don't get to 144 fps).
On the 60Hz monitor, you'll see 60 fps with a lot of tearing if VSync is disabled. Vsync would eliminate tearing without impacting visible FPS in this case