This depends on motherboard being used and PCIE slot configuration.
For example - motherboard may have 2 x16 PCIE slots. When one slot is used, it operates in x16 mode. When 2 slots are used, they switch to x8/x8 operation mode.
Also motherboard may have separate pcie lanes (not dirrectly connected to cpu). Those usually are x1, x2, x4 PCIE slots, but can also be x16 physical slot with less available connections.
So, to answer your question - would need to know motherboard model name, pcie devices you're trying to connect and slots to use.