no it doesn't. they could both be different versions of USB and you haven't even told us what you're trying to connect to them
Kind of overthinking it there. H81 haswell is not going to have a mix of USB 2.0 and 1.1 ports; and it is very clear that a USB 3.0 port cable does not fit in a usb 2.0 header.
Regardless if you looked at diagram for H81M-E33 board you would see that it only has usb 2.0 ports anyways.