I've already done that. So, the memory, although same spec, voltage and timings are most likely from different silicon. This is the key thing.
When you buy in a matched 2 x 8 or 2 x 16gb kit, those DIMMS have been tested to work together. This brings the QVL into line. The QVL is a list of ram that will work as a kit/pack od 2 or 4 DIMMS. If you buy separate DIMMs some time apart, the chances of them being on the same silicon is slim. This is where the DIMMs 'may' not work together.
By adding a single DIMM with the same specs, you are not guaranteed they will work together. You are only guaranteed it when they are from the matched kit. Most memory manufacturers will say this too.
As one of the other posters mentioned, they could work together flawlessly. But you won't know until you try. If they don't work, you know where to go.