First off, I'd avoid RAID0 as it is less reliable. You may not see much actual speed improvement in actual use depending on the use case either.
I'm not clear on what you're doing though. Just disconnecting the wires then plugging them back in? That affects nothing.
If for example you setup RAID0 at hardware level through the motherboard then using a different motherboard you might see the data unusable since it may not even recognize the array.
I'm less clear on what happens if the array is a SOFTWARE array setup through Windows but I'd never do that for RAID0.
To be clear, the data is SPLIT between both drives so if one drive fails or your array is not properly detected then everything is gone.