Hi Pres,
Some 3rd party PCI add in boards have drivers for NT4, so in theory at least the answer is YES!.
Microsoft's stand on the matter however is that NT 4 DOES NOT support USB. Hence many programs/support utils for USB devices detect NT4 as to OS and resfuse to even try and work, even though they would run.
As far as using the on-board USB ports go NT will not support them.
Regards
Simon