If it worked fine before you changed the IRQ sharing, then change it back. IRQs can be messy if you don't know what your doing. Think of all the things you use on your PC.
Mouse, keyboard, graphics, sound etc.
If they each have an IRQ this is fine. Some devices can share IRQs, but experimenting can cause major problems.
If it works, don't fix it.
Usually, the PCI slot nearest the centre of the mobo is slot 1. It can be worthwhile spacing cards out on the slots to allow ventilation.