will removing a ram stick cuases the mobo to reset overclicking settings?

Oct 3, 2018
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so i have a system with overclocked GPU and CPU and Ram.
does removing one of the ram stick (i have 4 of them) could cause the MOBO to reset the overclock's settings?
because i've noticed the system start to act weird (booting twice) but then it booted to windows just fine!

P.S: the system belongs to my brother and i dont want to mess around with it :)