Hey there, Jeremy!
Since the PC build is brand new with a new motherboard (I suppose) you need to buy a new genuine Windows installation. This is something that requires you to get in touch with
MS Support for further assistance. Either way, just like @Ambular mentioned you will need
a legitimate copy with a legitimate license key.
The operating system communicates with the motherboard. The HDD is just a carrier for the system files, you won't be able to boot into the OS that is on the drive from another PC.
Good luck!
SuperSoph_WD