You shouldn't use assistant fan header for case/radiator. No idea what it would do
, but rather save it as last option. If you use it (if connector is even compatible with assist fan header) make sure to check that fan in control software you got with your motherboard and set properly it's priorities for cooling, because motherboard will assume it's assist fan which is at specific location and will set it's priorities for cooling differently than you would like for example for case fan.
For your case if you lack connectors on board you should put 2 fans on one header (using Y splitter cable). So if you got for example 2 push fans on radiator put them on one header and if you got another 2 on pull put those 2 on second header. Motherboard should handle it np and both fans will be getting same signal = same speed.
Assistant fan is small 40mm fan which is bundled with your motherboard as I wrote it's meant to help airflow under thermal armor Sabertooth motherboard use. I got 2 such fans on my Z97 Sabertooth.