Does PCIe 2.0 effect usage of PCIe 3.0 lanes?

[A Furry Peanut]

So my CPU (i7-4790k) gives 16 lanes and my mobo (ASRock H97M PRO4) offers 1 PCIe 3.0 x16, 1 PCIe 2.0 x16, 2 PCI. My 1080 ti takes up 16 lanes of the PCIe 3.0. Would adding a Thunderbolt 2 AIC which takes up PCIe 2.0 x4 effect the performance of my 1080 ti with its PCIe 3.0 x16? If so, how much of an impact would it have? What if it was a thunderbolt 3 AIC which uses PCIe 3.0 x4?

I'm not sure which category this question belongs in, so apologies in advance if I placed it in the wrong one.

 

[SkyNetRising]

PCIE 3.0 x16 slot is connected to PCIE lanes provided by cpu,
PCIE 2.0 x16 is connected to chipset.
They do not impact one another.

 

[Dark Lord of Tech]

So my CPU (i7-4790k) gives 16 lanes and my mobo (ASRock H97M PRO4) offers 1 PCIe 3.0 x16, 1 PCIe 2.0 x16, 2 PCI. My 1080 ti takes up 16 lanes of the PCIe 3.0. Would adding a Thunderbolt 2 AIC which takes up PCIe 2.0 x4 effect the performance of my 1080 ti with its PCIe 3.0 x16? If so, how much of an impact would it have?
I'm not sure which category this question belongs in, so apologies in advance if I placed it in the wrong one.

It won't , your all set . no worries.

 

[Barty1884]

AND, even if the addition of an AIC limited your x16 slot, it would be to Gen3 x8 - and the performance impact sits somewhere between minimum and imperceptible

https://www.gamersnexus.net/guides/2488-pci-e-3-x8-vs-x16-performance-impact-on-gpus