Did you ever remove the GPU card from the slot during this whole process? At any point? If so, make sure it's fully seated and locked into place. Nothing AT ALL in Windows could have any effect on the GPU card not working BEFORE it begins booting into the windows environment. If the card was working there would be the bios post screen, at least, not a "no signal" screen.
Something has to have changed PHYSICALLY in order for it to have worked before but not now, at least prior to Windows starting to load. If you can't see anything on screen like bios or POST information, then something is physically not right. Double check cables, pins, reseat the card.
Might also try unplugging the PSU from the wall, with the power off of course, and then removing your CMOS battery from the motherboard for maybe two minutes, then reinstalling it, plug the PSU back in and start the system. See if there is still no signal.