Question Gpu into 2nd pcie 16 slot?

Jan 4, 2024
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Title is basically it. Can you plug in your gpu rtx card into the 2nd slot? Reason is it over take my other slots. If I can plug it in my 2nd one I'll have both of the others available for something else.. and I wouldnt block my gpus fans.
 
Both are 16x. Msi website states it for my board
In most of the motherboards, the first PCIE slot if the one that is directly connected to the CPU, to the other slots (even if they are 16x) they will lack a little bit than the first slot.

EDIT: also, the first slot is meant for a GPU specifically, thus it has every benefit over the other slots. there's nothing stopping you from connecting to the second slots, but be ready for the performance dip.