How does an SSD and an HDD work together?

[CeeTeePee]

I have a an SSD as my boot drive and an HDD as my storage. If I have, let's say, Microsoft word on my SSD (but not on my HDD) but my .docx file is on my HDD, will the file still run if I attempted to access it through my HDD?

 

[Mr Kagouris]

File location isn't an issue (obviously files will load slower on the HDD).