If you can get it to run, certainly.
I assume you have a 64 bit os capable of using more than 4gb.
Assuming you have 4 slots, put your current 2 sticks in one channel, and the 4gb stick in the other channel.
Both sticks will need to run at the same voltage, speed and timings.
It would not hurt to try, but working is not guaranteed. I put your odds at 80% success.
You might need to up the ram voltage a bit to compensate for the extra stick.