If you opened the case, removed the disks, handled them, then replaced them and re-assembled, you have a LOT more trouble than misaligned disks. I suspect you have actually connected the re-assembled drive to a computer and tried to use it, and that is how you know it does not work. That means it has rotated the disks, and probably the heads have moved in trying to access data. It is VERY likely that, by opening the case and handling the disks, you have added fine dust particles inside the case, and those can get caught under the heads and scratch the disk surfaces, ruining them.
As unksoil said, to have ANY hope of recovering some of your data (and maybe not all - depends if any areas of the disks have been ruined already), you will have to send it to a professional data recovery company that disassembles, cleans, and reassembles the unit. The cost is sure to be over $1000, probably a lot more.