I'm just recently bought a pcie wifi card from ASUS (asus pce-ac68) and I'm trying to use it with an Asrock b250m-hdv while the 16x slot is occupied by a GPU (GTX 1050). When the GPU was installed, the wifi card wouldn't appear anywhere in device manager or settings while it was in the x1 slot, however when I took out the GPU and put the wifi card into the 16x slot it worked. The only problem is I need the 16x slot for the GPU so I was wondering if the wifi card can be used in the x1 slot while the 16x is occupied.